Faecal microbiota transplantation-mediated jejunal microbiota changes halt high-fat diet-induced obesity in mice via retarding intestinal fat absorptionIF:3.5

microbiota-host interactions is an area that remains little
explored. M
6A has been ascertained to be the richest
modification existing on eukaryotic mRNA, which is clo-sely
associated with obesity (Roundtree et al., 2017; Wang et al.,
2020). Interestingly, the gut microbiota has been reported to
be involved in regulating m
6A levels in the gut, brain, liver
and other tissues (Wang et al., 2019; Jabs et al., 2020). We
found that wild boar faecal bacteria treatment alleviated the
decrease in jejunal m
6A levels induced by HFD, and the
results of correlation analysis also suggested that the
increase in Lactobacilli abun-dance after faecal bacteria
treatment was slightly posi-tively associated with m
6A levels.
Folate supplied by Lactobacillus or other bacteria could
promote RNA methy-lation by synthesizing the methyl donor
S-adenosylmethionine through the folate and methionine
cycle (Wu, Zhao, et al., 2020). We speculated that the
increase of Lactobacillus in the intestine caused by wild boar
faecal bacteria might play an indispensable role in regulating
the jejunal m
6A levels, and the significance of jejunal m
6A
levels may be the combined effect of multiple dominant
bacteria. These results implied that variations of intestinal
m
6A levels respond to the onset of obesity and the mitigating
effect of wild boar faecal bacteria on obe-sity. However,
further investigation is needed to explore the precise
mechanisms of gut microbiota regulating intestinal lipid
absorption and mRNA methylation level.

感谢农业农村部动物营养与饲料科学重点实验室（华东分所）引用文献