Gene and RNA Editing: Revolutionary Approaches to Treating Diseases

In base editing, the cytosine base editor (CBE) and adenine base
editor (ABE) have been applied in practice [33]. As the name
implies, the former converts the base C⋅G to G⋅A, while the latter
converts A-T to G-C [34]. Since both carry out single-base changes
without causing DSB, commonly used to correct point mutations
[35]. It is precisely because of this single base mutation that there
may be bystander editing, resulting in the editing of adjacent
bases as well [36]. PE is a gene editing method that combines
Cas9 nickase with reverse transcriptase and directly writes new
sequences through pegRNA. This method does not require DSB
or donor DNA and can perform various types of editing [37].
However, its design is complex and its overall efficiency is lower
than that of the standard CRISPR–Cas9 approach [38].
Gene technologies with different principles correspond to different gene editing tools. How to select appropriate gene editing
tools requires comparing the different characteristics of various
gene editing technologies and choosing the appropriate tools.

感谢中国上海海军医科大学心理学系引用文献