MYB30 transcription factor regulates oxidative and heat stress responses through ANNEXIN-mediated cytosolic calcium signaling in Arabidopsis

Cytosolic Ca2+ measurements
For AEQUORIN assays, 9-d-old seedlings growing on MS
medium were used for [Ca2+]cyt measurement. The [Ca2+]cyt
luminescence measurements were performed as described previously (Zhao et al., 2013), and the quantification of [Ca2+]cyt (inlmol l1) was performed as described by Rentel & Knight(2004). The plates were sprayed with 10 mM H2O2 and thephoton emissions were immediately collected for 3 min. For heattreatment, 30 ml of H2O at 45°C was poured into the plates andthe photon emissions were immediately collected for 8 min.For the Fluo-4 assay, the 7-d-old vertically cultured seedlings
of wild-type and myb30 were incubated with 10 lM Fluo-4-AMin the presence of 0.02% (v/v) pluronic F-127 for 2 h at 37°C.Fluorescence in the roots of stained seedlings was captured usingthe ANdor IQ confocal microscope (Andor, Belfast, NorthernIreland) (excitation at 488 nm and emission at 520 nm) afterstress treatments.

感谢中国农业大学生物科学学院植物生理与生物化学国家重点实验室引用文献